Method As per laboratory protocol. Rest Its and Discussion From the table and graph it shows that there is a linear relationship between concentration and absorption (Beer-Lambert Law). As the concentration increases so does the absorbency. The line of regression fits into the averages of the UP Absorbency, the RE is 0. 9538 this shows that the data is accurate as it is very close to the regression line. Taking the averages of the CIVIC Absorbency allows the data to become more accurate and reliable. Cavetti MM cuscus (ml) Distilled Water (m L)
Concentration (M) XIV Absorbency (Average) Dividing the unknown solutions by y is the way to find the concentrations of the unknown solutions. Plotting the unknown solutions On the graph Can test the reliability of the results of the concentration. The results are close to the line of regression, this indicates that the values which were calculated are accurate. The results which were gathered could have been skewed from the way the cavetti was placed into the spectrophotometer.
For instance, if the puttee had fingerprints on it, the wavelength would not penetrate the cavetti properly as the fingerprints may modify the way the light penetrates the solution, thus gathering an incorrect concentration reading. One way to avoid this is to clean the cavetti before placing it into the spectrophotometer, plus making sure that it is placed into the apparatus the right way. Whilst the 1 mol of Copper Sulfate was measured with the Gilson there may have been a small chance of the measurement being inaccurate, however, a Gilson is more likely to give an accurate measurement of a solution than a pipette old.
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When using a spectrophotometer it is essential that the apparatus is switched on and left to warm up for at least 15 minutes, if this is not executed and the experiment is done with a 'cold' spectrophotometer the readings may be unstable. Between each reading of the solutions the spectrophotometer transmittance needs to be set back to O, if this is not done the reading will be incorrect, therefore jeopardizing the accuracy and reliability of the results. Conclusion Within this practical the aim was achieved, the absorptions of the diluted solutions were determined and accurate.
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