Temperature and Its Effects on Respiration in Plants and Animals Introduction Cellular respiration is the process of breaking down organic compounds to create usable energy for plants and animals. Energy that results from this metabolic process is stored in the form of ATP (adenosine triphosphate) but carbon dioxide and water are also end products of this reaction. This makes it possible to study the amount of respiration of a plant or animal by measuring the rate at which carbon dioxide is released by the organism.
In this experiment, crickets and germinating seeds will be tested at three different temperature ranges and the carbon dioxide output measured and compared. Method To prepare the test, insert the CO? (carbon dioxide) probe into an empty respiration chamber and allow 90 seconds for the probe to warm up. Next, calibrate the CO? probe and allow 30 seconds for the CO? reading to be calculated and record the base reading. After the base reading has been taken weigh an empty respiration chamber in grams and then place 5 to 10 adult crickets, or 5 to 10 germinating seeds into the respiration chamber.
Record the weight once again with both the respiration chamber and the organisms combined. Now, subtract the weight of the empty respiration chamber from the weight of the organisms and the respiration chamber together to determine the mass of the crickets or seeds. Continue to prepare the test by placing the probe snugly onto the respiration chamber and ensure that all other holes are sealed. Begin to measure the CO? output in ppm (parts per million) at 10-15° C (ice bath), 20-25° C (room temperature) and 35-40° C (heated water bath. Allow five minutes for the temperature to stabilize when beginning to test a new temperature range and then proceed to collect data with the CO? probe. After a 3 minute period of data collection record the temperature inside the respiration chamber. Find the most linear part of the graph created from the data collected and determine the slope of the line. Divide the slope of the line by the mass of the crickets or seeds to determine the units in ppm/sec/g. Each temperature should be tested 2 to 3 times to get an average respiration rate for each temperature range tested. Hypothesis
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The test performed at the highest temperature will increase the rate of respiration of an organism, while the test conducted at the lowest temperature will decrease the rate of respiration of an organism. Results Respiration Rates at Various Temperature Ranges Respiration of Germinating Beans in ppm/sec/gRespiration of Crickets In ppm/sec/g TemperatureGroup 1Group 2Group 1Group 2 10-15°C0. 0020. 9231. 1430. 10 0. 18 1. 1790. 3830. 24 20-25°C0. 0960. 8940. 9630. 41 0. 2261. 0911. 1180. 50 35-40°C0. 273. 2552. 4621. 14 0. 473. 8662. 4771. 94 The rate of respiration in the germinating beans gradually increased as the temperature rose.
However, groups 1 and 2 studying the germinating beans collected some inconsistent data in the 20-25°C temperature range. The overall trend was similar in the data collected from the crickets’ respiration rates. They also respired at a greater rate when the temperature was elevated. Additionally, group 1 studying the crickets also recorded some erratic measurements in the 10-15°C temperature range. Conclusion The results of this test supported the hypothesis. It is clear that there is a direct relationship between temperature and respiration in plants and animals.
The tendency among both the germinating seeds and the crickets was an increase in respiration as the temperature intensified. The few inconsistencies that occurred throughout the test could have been attributed to not allowing sufficient time for the temperature to stabilize between testing different temperature ranges. Also, the CO? probe could have not been tightly sealed allowing oxygen to enter the respiration chamber and affecting the readings of CO? concentrations. When repeating this test it would be important to ensure that these errors are more closely controlled or corrected so that accurate readings could be recorded and evaluated.
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